WORKSHOP: Single cell RNAseq analysis in R
This record includes training materials associated with the Australian BioCommons workshop 'Single cell RNAseq analysis in R'. This workshop took place over two, 3.5 hour sessions on 26 and 27 October 2023.Event descriptionAnalysis and interpretation of single cell RNAseq (scRNAseq) data requires...
Keywords: bioinformatics, transcriptomics, single cell RNA-seq, Seurat, R statistical software
WORKSHOP: Single cell RNAseq analysis in R
https://zenodo.org/records/10042919
https://dresa.org.au/materials/workshop-single-cell-rnaseq-analysis-in-r-6a1126cf-7105-43ec-bf55-7c492f758301
This record includes training materials associated with the Australian BioCommons workshop 'Single cell RNAseq analysis in R'. This workshop took place over two, 3.5 hour sessions on 26 and 27 October 2023.Event descriptionAnalysis and interpretation of single cell RNAseq (scRNAseq) data requires dedicated workflows. In this hands-on workshop we will show you how to perform single cell analysis using Seurat - an R package for QC, analysis, and exploration of single-cell RNAseq data. We will discuss the 'why' behind each step and cover reading in the count data, quality control, filtering, normalisation, clustering, UMAP layout and identification of cluster markers. We will also explore various ways of visualising single cell expression data.This workshop is presented by the Australian BioCommons, Queensland Cyber Infrastructure Foundation (QCIF) and the Monash Genomics and Bioinformatics Platform with the assistance of a network of facilitators from the national Bioinformatics Training Cooperative.Lead trainers: Sarah Williams, Adele Barugahare, Paul Harrison, Laura Perlaza JimenezFacilitators: Nick Matigan, Valentine Murigneux, Magdalena (Magda) AntczakInfrastructure provision: Uwe WinterCoordinator: Melissa BurkeTraining materialsMaterials are shared under a Creative Commons Attribution 4.0 International agreement unless otherwise specified and were current at the time of the event.Files and materials included in this record:Event metadata (PDF): Information about the event including, description, event URL, learning objectives, prerequisites, technical requirements etc.Index of training materials (PDF): List and description of all materials associated with this event including the name, format, location and a brief description of each file.scRNAseq_Schedule (PDF): A breakdown of the topics and timings for the workshopMaterials shared elsewhere:This workshop follows the tutorial 'scRNAseq Analysis in R with Seurat'https://swbioinf.github.io/scRNAseqInR_Doco/index.htmlSlides used to introduce key topics are available via GitHubhttps://github.com/swbioinf/scRNAseqInR_Doco/tree/main/slidesThis material is based on the introductory Guided Clustering Tutorial tutorial from Seurat.It is also drawing from a similar workshop held by Monash Bioinformatics Platform Single-Cell-Workshop, with material here.
Melissa Burke (melissa@biocommons.org.au)
Williams, Sarah
Barugahare, Adele (orcid: 0000-0002-8976-0094)
Harrison, Paul (orcid: 0000-0002-3980-268X)
Perlaza Jimenez, Laura (orcid: 0000-0002-8511-1134)
Matigan, Nicholas
Murigneux, Valentine (orcid: 0000-0002-1235-9462)
Antczak, Magdalena (orcid: 0000-0003-1503-1849)
Winter, Uwe
bioinformatics, transcriptomics, single cell RNA-seq, Seurat, R statistical software
WORKSHOP: Single cell RNAseq analysis in R
This record includes training materials associated with the Australian BioCommons workshop ‘Single cell RNAseq analysis in R’. This workshop took place over two, 3.5 hour sessions on 22 and 3 August 2022.
Event description
Analysis and interpretation of single cell RNAseq (scRNAseq) data...
Keywords: Bioinformatics, Analysis, Transcriptomics, R software, Single cell RNAseq, scRNAseq
WORKSHOP: Single cell RNAseq analysis in R
https://zenodo.org/records/7072910
https://dresa.org.au/materials/workshop-single-cell-rnaseq-analysis-in-r-4f60b82d-2f1e-4021-9569-6955878dd945
This record includes training materials associated with the Australian BioCommons workshop ‘Single cell RNAseq analysis in R’. This workshop took place over two, 3.5 hour sessions on 22 and 3 August 2022.
Event description
Analysis and interpretation of single cell RNAseq (scRNAseq) data requires dedicated workflows. In this hands-on workshop we will show you how to perform single cell analysis using Seurat - an R package for QC, analysis, and exploration of single-cell RNAseq data.
We will discuss the ‘why’ behind each step and cover reading in the count data, quality control, filtering, normalisation, clustering, UMAP layout and identification of cluster markers. We will also explore various ways of visualising single cell expression data.
This workshop is presented by the Australian BioCommons and Queensland Cyber Infrastructure Foundation (QCIF) with the assistance of a network of facilitators from the national Bioinformatics Training Cooperative.
Materials are shared under a Creative Commons Attribution 4.0 International agreement unless otherwise specified and were current at the time of the event.
Files and materials included in this record:
Event metadata (PDF): Information about the event including, description, event URL, learning objectives, prerequisites, technical requirements etc.
Index of training materials (PDF): List and description of all materials associated with this event including the name, format, location and a brief description of each file.
scRNAseq_Slides (PDF): Slides used to introduce topics
scRNAseq_Schedule (PDF): A breakdown of the topics and timings for the workshop
scRNAseq_Resources (PDF): A list of resources recommended by trainers and participants
scRNAseq_QandA(PDF): Archive of questions and their answers from the workshop Slack Channel.
Materials shared elsewhere:
This workshop follows the tutorial ‘scRNAseq Analysis in R with Seurat’
https://swbioinf.github.io/scRNAseqInR_Doco/index.html
This material is based on the introductory Guided Clustering Tutorial tutorial from Seurat.
It is also drawing from a similar workshop held by Monash Bioinformatics Platform Single-Cell-Workshop, with material here.
Melissa Burke (melissa@biocommons.org.au)
Williams, Sarah
Mehdi, Ahmed (orcid: 0000-0002-9300-2341)
Matigan, Nick
Barugahare, Adele (orcid: 0000-0002-8976-0094)
Harrison, Paul (orcid: 0000-0002-3980-268X)
Morgan, Steven (orcid: 0000-0001-6038-6126)
Whitfield, Holly (orcid: 0000-0002-7282-387X)
Bioinformatics, Analysis, Transcriptomics, R software, Single cell RNAseq, scRNAseq
WORKSHOP: Working with genomics sequences and features in R with Bioconductor
This record includes training materials associated with the Australian BioCommons workshop ‘Working with genomics sequences and features in R with Bioconductor’. This workshop took place on 23 September 2021.
Workshop description
Explore the many useful functions that the Bioconductor...
Keywords: R software, Bioconductor, Bioinformatics, Analysis, Genomics, Sequence analysis
WORKSHOP: Working with genomics sequences and features in R with Bioconductor
https://zenodo.org/records/5781776
https://dresa.org.au/materials/workshop-working-with-genomics-sequences-and-features-in-r-with-bioconductor-8399bf0d-1e9e-48f3-a840-3f70f23254bb
This record includes training materials associated with the Australian BioCommons workshop ‘Working with genomics sequences and features in R with Bioconductor’. This workshop took place on 23 September 2021.
Workshop description
Explore the many useful functions that the Bioconductor environment offers for working with genomic data and other biological sequences.
DNA and proteins are often represented as files containing strings of nucleic acids or amino acids. They are associated with text files that provide additional contextual information such as genome annotations.
This workshop provides hands-on experience with tools, software and packages available in R via Bioconductor for manipulating, exploring and extracting information from biological sequences and annotation files. We will look at tools for working with some commonly used file formats including FASTA, GFF3, GTF, methods for identifying regions of interest, and easy methods for obtaining data packages such as genome assemblies.
This workshop is presented by the Australian BioCommons and Monash Bioinformatics Platform with the assistance of a network of facilitators from the national Bioinformatics Training Cooperative.
Materials are shared under a Creative Commons Attribution 4.0 International agreement unless otherwise specified and were current at the time of the event.
Files and materials included in this record:
Event metadata (PDF): Information about the event including, description, event URL, learning objectives, prerequisites, technical requirements etc.
Index of training materials (PDF): List and description of all materials associated with this event including the name, format, location and a brief description of each file.
Schedule (PDF): schedule for the workshop providing a breakdown of topics and timings
Materials shared elsewhere:
This workshop follows the tutorial ‘Working with DNA sequences and features in R with Bioconductor - version 2’ developed for Monash Bioinformatics Platform and Monash Data Fluency by Paul Harrison.
https://monashdatafluency.github.io/r-bioc-2/
Melissa Burke (melissa@biocommons.org.au)
Harrison, Paul (orcid: 0000-0002-3980-268X)
Deshpande, Nandan (orcid: 0000-0002-0324-8728)
Barugahare, Adele (orcid: 0000-0002-8976-0094)
Perry, Andrew (orcid: 0000-0001-9256-6068)
Wong, Nick (orcid: 0000-0003-4393-7541)
Reames, Benjamin
R software, Bioconductor, Bioinformatics, Analysis, Genomics, Sequence analysis
Deep Learning for Natural Language Processing
This workshop is designed to be instructor led and consists of two parts.
Part 1 consists of a lecture-demo about text processing and a hands-on session for attendees to learn how to clean a dataset.
Part 2 consists of a lecture introducing Recurrent Neural Networks and a hands-on session for...
Keywords: Deep learning, NLP, Machine learning
Resource type: presentation, tutorial
Deep Learning for Natural Language Processing
https://doi.org/10.26180/13100513
https://dresa.org.au/materials/deep-learning-for-natural-language-processing
This workshop is designed to be instructor led and consists of two parts.
Part 1 consists of a lecture-demo about text processing and a hands-on session for attendees to learn how to clean a dataset.
Part 2 consists of a lecture introducing Recurrent Neural Networks and a hands-on session for attendees to train their own RNN.
The Powerpoints contain the lecture slides, while the Jupyter notebooks (.ipynb) contain the hands-on coding exercises.
This workshop introduces natural language as data for deep learning. We discuss various techniques and software packages (e.g. python strings, RegEx, NLTK, Word2Vec) that help us convert, clean, and formalise text data “in the wild” for use in a deep learning model. We then explore the training and testing of a Recurrent Neural Network on the data to complete a real world task. We will be using TensorFlow v2 for this purpose.
datascienceplatform@monash.edu
Titus Tang
Deep learning, NLP, Machine learning
Getting Started with Deep Learning
This lecture provides a high level overview of how you could get started with developing deep learning applications. It introduces deep learning in a nutshell and then provides advice relating to the concepts and skill sets you would need to know and have in order to build a deep learning...
Keywords: Deep learning, Machine learning
Resource type: presentation
Getting Started with Deep Learning
https://doi.org/10.26180/15032688
https://dresa.org.au/materials/getting-started-with-deep-learning
This lecture provides a high level overview of how you could get started with developing deep learning applications. It introduces deep learning in a nutshell and then provides advice relating to the concepts and skill sets you would need to know and have in order to build a deep learning application. The lecture also provides pointers to various resources you could use to gain a stronger foothold in deep learning.
This lecture is targeted at researchers who may be complete beginners in machine learning, deep learning, or even with programming, but who would like to get into the space to build AI systems hands-on.
datascienceplatform@monash.edu
Titus Tang
Deep learning, Machine learning
Semi-Supervised Deep Learning
Modern deep neural networks require large amounts of labelled data to train. Obtaining the required labelled data is often an expensive and time consuming process. Semi-supervised deep learning involves the use of various creative techniques to train deep neural networks on partially labelled...
Keywords: Deep learning, Machine learning, semi-supervised
Resource type: presentation, tutorial
Semi-Supervised Deep Learning
https://doi.org/10.26180/14176805
https://dresa.org.au/materials/semi-supervised-deep-learning
Modern deep neural networks require large amounts of labelled data to train. Obtaining the required labelled data is often an expensive and time consuming process. Semi-supervised deep learning involves the use of various creative techniques to train deep neural networks on partially labelled data. If successful, it allows better training of a model despite the limited amount of labelled data available.
This workshop is designed to be instructor led and covers various semi-supervised learning techniques available in the literature. The workshop consists of a lecture introducing at a high level a selection of techniques that are suitable for semi-supervised deep learning. We discuss how these techniques can be implemented and the underlying assumptions they require. The lecture is followed by a hands-on session where attendees implement a semi-supervised learning technique to train a neural network. We observe and discuss the changing performance and behaviour of the network as varying degrees of labelled and unlabelled data is provided to the network during training.
datascienceplatform@monash.edu
Titus Tang
Deep learning, Machine learning, semi-supervised
Introduction to Deep Learning and TensorFlow
This workshop is intended to run as an instructor guided live event and consists of two parts. Each part consists of a lecture and a hands-on coding exercise.
Part 1 - Introduction to Deep Learning and TensorFlow
Part 2 - Introduction to Convolutional Neural Networks
The Powerpoints contain...
Keywords: Deep learning, convolutional neural network, tensorflow, Machine learning
Resource type: presentation, tutorial
Introduction to Deep Learning and TensorFlow
https://doi.org/10.26180/13100519
https://dresa.org.au/materials/introduction-to-deep-learning-and-tensorflow
This workshop is intended to run as an instructor guided live event and consists of two parts. Each part consists of a lecture and a hands-on coding exercise.
Part 1 - Introduction to Deep Learning and TensorFlow
Part 2 - Introduction to Convolutional Neural Networks
The Powerpoints contain the lecture slides, while the Jupyter notebooks (.ipynb) contain the hands-on coding exercises.
This workshop is an introduction to how deep learning works and how you could create a neural network using TensorFlow v2. We start by learning the basics of deep learning including what a neural network is, how information passes through the network, and how the network learns from data through the automated process of gradient descent. Workshop attendees would build, train and evaluate a neural network using a cloud GPU (Google Colab).
In part 2, we look at image data and how we could train a convolution neural network to classify images. Workshop attendees will extend their knowledge from the first part to design, train and evaluate this convolutional neural network.
datascienceplatform@monash.edu
Titus Tang
Deep learning, convolutional neural network, tensorflow, Machine learning