WORKSHOP: Single cell RNAseq analysis in R
This record includes training materials associated with the Australian BioCommons workshop 'Single cell RNAseq analysis in R'. This workshop took place over two, 3.5 hour sessions on 26 and 27 October 2023.Event descriptionAnalysis and interpretation of single cell RNAseq (scRNAseq) data requires...
Keywords: bioinformatics, transcriptomics, single cell RNA-seq, Seurat, R statistical software
WORKSHOP: Single cell RNAseq analysis in R
https://zenodo.org/records/10042919
https://dresa.org.au/materials/workshop-single-cell-rnaseq-analysis-in-r-6a1126cf-7105-43ec-bf55-7c492f758301
This record includes training materials associated with the Australian BioCommons workshop 'Single cell RNAseq analysis in R'. This workshop took place over two, 3.5 hour sessions on 26 and 27 October 2023.Event descriptionAnalysis and interpretation of single cell RNAseq (scRNAseq) data requires dedicated workflows. In this hands-on workshop we will show you how to perform single cell analysis using Seurat - an R package for QC, analysis, and exploration of single-cell RNAseq data. We will discuss the 'why' behind each step and cover reading in the count data, quality control, filtering, normalisation, clustering, UMAP layout and identification of cluster markers. We will also explore various ways of visualising single cell expression data.This workshop is presented by the Australian BioCommons, Queensland Cyber Infrastructure Foundation (QCIF) and the Monash Genomics and Bioinformatics Platform with the assistance of a network of facilitators from the national Bioinformatics Training Cooperative.Lead trainers: Sarah Williams, Adele Barugahare, Paul Harrison, Laura Perlaza JimenezFacilitators: Nick Matigan, Valentine Murigneux, Magdalena (Magda) AntczakInfrastructure provision: Uwe WinterCoordinator: Melissa BurkeTraining materialsMaterials are shared under a Creative Commons Attribution 4.0 International agreement unless otherwise specified and were current at the time of the event.Files and materials included in this record:Event metadata (PDF): Information about the event including, description, event URL, learning objectives, prerequisites, technical requirements etc.Index of training materials (PDF): List and description of all materials associated with this event including the name, format, location and a brief description of each file.scRNAseq_Schedule (PDF): A breakdown of the topics and timings for the workshopMaterials shared elsewhere:This workshop follows the tutorial 'scRNAseq Analysis in R with Seurat'https://swbioinf.github.io/scRNAseqInR_Doco/index.htmlSlides used to introduce key topics are available via GitHubhttps://github.com/swbioinf/scRNAseqInR_Doco/tree/main/slidesThis material is based on the introductory Guided Clustering Tutorial tutorial from Seurat.It is also drawing from a similar workshop held by Monash Bioinformatics Platform Single-Cell-Workshop, with material here.
Melissa Burke (melissa@biocommons.org.au)
Williams, Sarah
Barugahare, Adele (orcid: 0000-0002-8976-0094)
Harrison, Paul (orcid: 0000-0002-3980-268X)
Perlaza Jimenez, Laura (orcid: 0000-0002-8511-1134)
Matigan, Nicholas
Murigneux, Valentine (orcid: 0000-0002-1235-9462)
Antczak, Magdalena (orcid: 0000-0003-1503-1849)
Winter, Uwe
bioinformatics, transcriptomics, single cell RNA-seq, Seurat, R statistical software
WORKSHOP: Single cell RNAseq analysis in R
This record includes training materials associated with the Australian BioCommons workshop ‘Single cell RNAseq analysis in R’. This workshop took place over two, 3.5 hour sessions on 22 and 3 August 2022.
Event description
Analysis and interpretation of single cell RNAseq (scRNAseq) data...
Keywords: Bioinformatics, Analysis, Transcriptomics, R software, Single cell RNAseq, scRNAseq
WORKSHOP: Single cell RNAseq analysis in R
https://zenodo.org/records/7072910
https://dresa.org.au/materials/workshop-single-cell-rnaseq-analysis-in-r-4f60b82d-2f1e-4021-9569-6955878dd945
This record includes training materials associated with the Australian BioCommons workshop ‘Single cell RNAseq analysis in R’. This workshop took place over two, 3.5 hour sessions on 22 and 3 August 2022.
Event description
Analysis and interpretation of single cell RNAseq (scRNAseq) data requires dedicated workflows. In this hands-on workshop we will show you how to perform single cell analysis using Seurat - an R package for QC, analysis, and exploration of single-cell RNAseq data.
We will discuss the ‘why’ behind each step and cover reading in the count data, quality control, filtering, normalisation, clustering, UMAP layout and identification of cluster markers. We will also explore various ways of visualising single cell expression data.
This workshop is presented by the Australian BioCommons and Queensland Cyber Infrastructure Foundation (QCIF) with the assistance of a network of facilitators from the national Bioinformatics Training Cooperative.
Materials are shared under a Creative Commons Attribution 4.0 International agreement unless otherwise specified and were current at the time of the event.
Files and materials included in this record:
Event metadata (PDF): Information about the event including, description, event URL, learning objectives, prerequisites, technical requirements etc.
Index of training materials (PDF): List and description of all materials associated with this event including the name, format, location and a brief description of each file.
scRNAseq_Slides (PDF): Slides used to introduce topics
scRNAseq_Schedule (PDF): A breakdown of the topics and timings for the workshop
scRNAseq_Resources (PDF): A list of resources recommended by trainers and participants
scRNAseq_QandA(PDF): Archive of questions and their answers from the workshop Slack Channel.
Materials shared elsewhere:
This workshop follows the tutorial ‘scRNAseq Analysis in R with Seurat’
https://swbioinf.github.io/scRNAseqInR_Doco/index.html
This material is based on the introductory Guided Clustering Tutorial tutorial from Seurat.
It is also drawing from a similar workshop held by Monash Bioinformatics Platform Single-Cell-Workshop, with material here.
Melissa Burke (melissa@biocommons.org.au)
Williams, Sarah
Mehdi, Ahmed (orcid: 0000-0002-9300-2341)
Matigan, Nick
Barugahare, Adele (orcid: 0000-0002-8976-0094)
Harrison, Paul (orcid: 0000-0002-3980-268X)
Morgan, Steven (orcid: 0000-0001-6038-6126)
Whitfield, Holly (orcid: 0000-0002-7282-387X)
Bioinformatics, Analysis, Transcriptomics, R software, Single cell RNAseq, scRNAseq
WORKSHOP: Working with genomics sequences and features in R with Bioconductor
This record includes training materials associated with the Australian BioCommons workshop ‘Working with genomics sequences and features in R with Bioconductor’. This workshop took place on 23 September 2021.
Workshop description
Explore the many useful functions that the Bioconductor...
Keywords: R software, Bioconductor, Bioinformatics, Analysis, Genomics, Sequence analysis
WORKSHOP: Working with genomics sequences and features in R with Bioconductor
https://zenodo.org/records/5781776
https://dresa.org.au/materials/workshop-working-with-genomics-sequences-and-features-in-r-with-bioconductor-8399bf0d-1e9e-48f3-a840-3f70f23254bb
This record includes training materials associated with the Australian BioCommons workshop ‘Working with genomics sequences and features in R with Bioconductor’. This workshop took place on 23 September 2021.
Workshop description
Explore the many useful functions that the Bioconductor environment offers for working with genomic data and other biological sequences.
DNA and proteins are often represented as files containing strings of nucleic acids or amino acids. They are associated with text files that provide additional contextual information such as genome annotations.
This workshop provides hands-on experience with tools, software and packages available in R via Bioconductor for manipulating, exploring and extracting information from biological sequences and annotation files. We will look at tools for working with some commonly used file formats including FASTA, GFF3, GTF, methods for identifying regions of interest, and easy methods for obtaining data packages such as genome assemblies.
This workshop is presented by the Australian BioCommons and Monash Bioinformatics Platform with the assistance of a network of facilitators from the national Bioinformatics Training Cooperative.
Materials are shared under a Creative Commons Attribution 4.0 International agreement unless otherwise specified and were current at the time of the event.
Files and materials included in this record:
Event metadata (PDF): Information about the event including, description, event URL, learning objectives, prerequisites, technical requirements etc.
Index of training materials (PDF): List and description of all materials associated with this event including the name, format, location and a brief description of each file.
Schedule (PDF): schedule for the workshop providing a breakdown of topics and timings
Materials shared elsewhere:
This workshop follows the tutorial ‘Working with DNA sequences and features in R with Bioconductor - version 2’ developed for Monash Bioinformatics Platform and Monash Data Fluency by Paul Harrison.
https://monashdatafluency.github.io/r-bioc-2/
Melissa Burke (melissa@biocommons.org.au)
Harrison, Paul (orcid: 0000-0002-3980-268X)
Deshpande, Nandan (orcid: 0000-0002-0324-8728)
Barugahare, Adele (orcid: 0000-0002-8976-0094)
Perry, Andrew (orcid: 0000-0001-9256-6068)
Wong, Nick (orcid: 0000-0003-4393-7541)
Reames, Benjamin
R software, Bioconductor, Bioinformatics, Analysis, Genomics, Sequence analysis
Data Policy
Increasing the availability of research data for reuse is in part being driven by research data policies. While the number of research funders, journals and institutions with some form of research data policy is growing, the landscape is complex and therefore the implementation and implications...
Keywords: policy, data policy, publishers, training material
Data Policy
https://zenodo.org/records/4922756
https://dresa.org.au/materials/data-policy-c8bc856f-0afa-49dc-b100-36e9a8375327
Increasing the availability of research data for reuse is in part being driven by research data policies. While the number of research funders, journals and institutions with some form of research data policy is growing, the landscape is complex and therefore the implementation and implications of policies for researchers can be unclear, confusing and sometimes even contradictory. The RDA Data Policy Standardisation and Implementation IG was established to help address these challenges.
Initially the Group focussed on Developing a Research Data Policy Framework for All Journals and Publishers and with journal adoptions of the framework growing, the Group is now focussing on alignment between publishers and funders. This session provided an overview of the joint session held at RDA P17 of the Research Funders and Stakeholders on Open Research and Data Management and Practices IG, the Data Policy Standardisation and Implementation IG and the FAIRsharing WG.
The focus of this RDA VP17 session was to provide an overview of a joint project to examine funder-publisher policy alignment and provide recommendations on how to improve alignment.
contact@ardc.edu.au
Simons, Natasha (orcid: 0000-0003-0635-1998)
policy, data policy, publishers, training material
ARDC FAIR Data 101 self-guided
FAIR Data 101 v3.0 is a self-guided course covering the FAIR Data principles
The FAIR Data 101 virtual course was designed and delivered by the ARDC Skilled Workforce Program twice in 2020 and has now been reworked as a self-guided course.
The course structure was based on 'FAIR Data in the...
Keywords: training material, FAIR data, video, webinar, activities, quiz, FAIR, research data management
ARDC FAIR Data 101 self-guided
https://zenodo.org/records/5094034
https://dresa.org.au/materials/ardc-fair-data-101-self-guided-2d794a84-f0ff-4e11-a39c-fa8ea481e097
FAIR Data 101 v3.0 is a self-guided course covering the FAIR Data principles
The FAIR Data 101 virtual course was designed and delivered by the ARDC Skilled Workforce Program twice in 2020 and has now been reworked as a self-guided course.
The course structure was based on 'FAIR Data in the Scholarly Communications Lifecycle', run by Natasha Simons at the FORCE11 Scholarly Communications Institute. These training materials are hosted on GitHub.
contact@ardc.edu.au
Stokes, Liz (orcid: 0000-0002-2973-5647)
Liffers, Matthias (orcid: 0000-0002-3639-2080)
Burton, Nichola (orcid: 0000-0003-4470-4846)
Martinez, Paula A. (orcid: 0000-0002-8990-1985)
Simons, Natasha (orcid: 0000-0003-0635-1998)
Russell, Keith (orcid: 0000-0001-5390-2719)
McCafferty, Siobhann (orcid: 0000-0002-2491-0995)
Ferrers, Richard (orcid: 0000-0002-2923-9889)
McEachern, Steve (orcid: 0000-0001-7848-4912)
Barlow, Melanie (orcid: 0000-0002-3956-5784)
Brady, Catherine (orcid: 0000-0002-7919-7592)
Brownlee, Rowan (orcid: 0000-0002-1955-1262)
Honeyman, Tom (orcid: 0000-0001-9448-4023)
Quiroga, Maria del Mar (orcid: 0000-0002-8943-2808)
training material, FAIR data, video, webinar, activities, quiz, FAIR, research data management