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Authors: Barlow, Melanie (orcid: 000...  or Chung, Jessica (orcid: 0000...  or Chandrananda, Dineika (orci...  or Marlies Hankel 

WORKSHOP: RNASeq: reads to differential genes and pathways

This record includes training materials associated with the Australian BioCommons workshop 'RNASeq: reads to differential genes and pathways'. This workshop took place over two, 3 hour sessions on 11 and 12 October 2023.Event descriptionRNA sequencing (RNAseq) is a popular and powerful technique...

Keywords: bioinformatics, transcriptomics, RNA-seq, RNAseq

WORKSHOP: RNASeq: reads to differential genes and pathways https://dresa.org.au/materials/workshop-rnaseq-reads-to-differential-genes-and-pathways This record includes training materials associated with the Australian BioCommons workshop 'RNASeq: reads to differential genes and pathways'. This workshop took place over two, 3 hour sessions on 11 and 12 October 2023.Event descriptionRNA sequencing (RNAseq) is a popular and powerful technique used to understand the activity of genes. Using differential gene profiling methods, we can use RNAseq data to gain valuable insights into gene activity and identify variability in gene expression between samples to understand the molecular pathways underpinning many different traits.  In this hands-on workshop, you will learn RNAseq fundamentals as you process, analyse, and interpret the results from a real RNAseq experiment on the command-line. In session one, you will convert raw sequence reads to analysis-ready count data with the nf-core/rnaseq workflow. In session two, you'll work interactively in RStudio to identify differentially expressed genes,perform functional enrichment analysis, and visualise and interpret your results using popular and best practice R packages.  This workshop was delivered as a part of the Australian BioCommons Bring Your Own Data Platforms Project and will provide you with an opportunity to explore services and infrastructure built specifically for life scientists working at the command line. By the end of the workshop, you will be familiar with Pawsey's Nimbus cloud platform and be able to process your own RNAseq datasets and perform differential expression analysis on the command-line. Materials are shared under a Creative Commons Attribution 4.0 International agreement unless otherwise specified and were current at the time of the event.Lead trainers: Dr Georgina Samaha (Sydney Informatics Hub), Dr Nandan Deshpande (Sydney Informatics Hub)Facilitators: Ching-Yu Lu and Jessica Chung.Infrastructure provision: Audrey Stott (Pawsey Supercomputing Research Centre), Alex Ip (AARNet)Host: Melissa Burke, Australian BioCommons Training materialsFiles and materials included in this record:Event metadata (PDF): Information about the event including, description, event URL, learning objectives, prerequisites, technical requirements etc.Index of training materials (PDF): List and description of all materials associated with this event including the name, format, location and a brief description of each file.Materials shared elsewhere:This workshop follows the tutorial 'Introduction to RNAseq workshop: reads to differential gene expression' developed by the Sydney Informatics Hub.https://sydney-informatics-hub.github.io/rnaseq-workshop-2023/Additional supporting materials are available via GitHubRstudio rnaseq container: https://github.com/Sydney-Informatics-Hub/Rstudio-rnaseq-contained/tree/mainRNAseq differential expression R notebook: https://github.com/Sydney-Informatics-Hub/rna-differential-expression-Rnotebook Melissa Burke (melissa@biocommons.org.au) bioinformatics, transcriptomics, RNA-seq, RNAseq
WORKSHOP: R: fundamental skills for biologists

This record includes training materials associated with the Australian BioCommons workshop ‘R: fundamental skills for biologists’. This workshop took place over four, three-hour sessions on 1, 8, 15 and 22 June 2022.

 

Event description

Biologists need data analysis skills to be able to...

Keywords: Bioinformatics, Analysis, Statistics, R software, RStudio, Data visualisation

WORKSHOP: R: fundamental skills for biologists https://dresa.org.au/materials/workshop-r-fundamental-skills-for-biologists-81aa00db-63ad-4962-a7ac-b885bf9f676b This record includes training materials associated with the Australian BioCommons workshop ‘R: fundamental skills for biologists’. This workshop took place over four, three-hour sessions on 1, 8, 15 and 22 June 2022.   Event description Biologists need data analysis skills to be able to interpret, visualise and communicate their research results. While Excel can cover some data analysis needs, there is a better choice, particularly for large and complex datasets.  R is a free, open-source software and programming language that enables data exploration, statistical analysis, visualisation and more. The large variety of R packages available for analysing biological data make it a robust and flexible option for data of all shapes and sizes.  Getting started can be a little daunting for those without a background in statistics and programming. In this workshop we will equip you with the foundations for getting the most out of R and RStudio, an interactive way of structuring and keeping track of your work in R. Using biological data from a model of influenza infection, you will learn how to efficiently and reproducibly organise, read, wrangle, analyse, visualise and generate reports from your data in R. Topics covered in this workshop include: Spreadsheets, organising data and first steps with R Manipulating and analysing data with dplyr Data visualisation Summarized experiments and getting started with Bioconductor   This workshop is presented by the Australian BioCommons and Saskia Freytag from WEHI  with the assistance of a network of facilitators from the national Bioinformatics Training Cooperative. Materials are shared under a Creative Commons Attribution 4.0 International agreement unless otherwise specified and were current at the time of the event. Files and materials included in this record: Event metadata (PDF): Information about the event including, description, event URL, learning objectives, prerequisites, technical requirements etc. Index of training materials (PDF): List and description of all materials associated with this event including the name, format, location and a brief description of each file. Schedule (PDF): A breakdown of the topics and timings for the workshop Recommended resources (PDF): A list of resources recommended by trainers and participants Q_and_A(PDF): Archive of questions and their answers from the workshop Slack Channel. Materials shared elsewhere:   This workshop follows the tutorial ‘Introduction to data analysis with R and Bioconductor’ which is publicly available. https://saskiafreytag.github.io/biocommons-r-intro/ This is derived from material produced as part of The Carpentries Incubator project https://carpentries-incubator.github.io/bioc-intro/ Melissa Burke (melissa@biocommons.org.au) Bioinformatics, Analysis, Statistics, R software, RStudio, Data visualisation
ARDC FAIR Data 101 self-guided

FAIR Data 101 v3.0 is a self-guided course covering the FAIR Data principles

The FAIR Data 101 virtual course was designed and delivered by the ARDC Skilled Workforce Program twice in 2020 and has now been reworked as a self-guided course.

The course structure was based on 'FAIR Data in the...

Keywords: training material, FAIR data, video, webinar, activities, quiz, FAIR, research data management

ARDC FAIR Data 101 self-guided https://dresa.org.au/materials/ardc-fair-data-101-self-guided-2d794a84-f0ff-4e11-a39c-fa8ea481e097 FAIR Data 101 v3.0 is a self-guided course covering the FAIR Data principles The FAIR Data 101 virtual course was designed and delivered by the ARDC Skilled Workforce Program twice in 2020 and has now been reworked as a self-guided course. The course structure was based on 'FAIR Data in the Scholarly Communications Lifecycle', run by Natasha Simons at the FORCE11 Scholarly Communications Institute. These training materials are hosted on GitHub. contact@ardc.edu.au training material, FAIR data, video, webinar, activities, quiz, FAIR, research data management
WORKSHOP: R: fundamental skills for biologists

This record includes training materials associated with the Australian BioCommons workshop ‘R: fundamental skills for biologists’. This workshop took place over four, three-hour sessions on 1, 8, 15 and 22 June 2022.

Event description

Biologists need data analysis skills to be able to...

Keywords: Bioinformatics, Analysis, Statistics, R software, RStudio, Data visualisation

WORKSHOP: R: fundamental skills for biologists https://dresa.org.au/materials/workshop-r-fundamental-skills-for-biologists This record includes training materials associated with the Australian BioCommons workshop ‘R: fundamental skills for biologists’. This workshop took place over four, three-hour sessions on 1, 8, 15 and 22 June 2022. **Event description** Biologists need data analysis skills to be able to interpret, visualise and communicate their research results. While Excel can cover some data analysis needs, there is a better choice, particularly for large and complex datasets.  R is a free, open-source software and programming language that enables data exploration, statistical analysis, visualisation and more. The large variety of R packages available for analysing biological data make it a robust and flexible option for data of all shapes and sizes.  Getting started can be a little daunting for those without a background in statistics and programming. In this workshop we will equip you with the foundations for getting the most out of R and RStudio, an interactive way of structuring and keeping track of your work in R. Using biological data from a model of influenza infection, you will learn how to efficiently and reproducibly organise, read, wrangle, analyse, visualise and generate reports from your data in R. Topics covered in this workshop include: - Spreadsheets, organising data and first steps with R - Manipulating and analysing data with dplyr - Data visualisation - Summarized experiments and getting started with Bioconductor This workshop is presented by the Australian BioCommons and Saskia Freytag from WEHI  with the assistance of a network of facilitators from the national Bioinformatics Training Cooperative. Materials are shared under a Creative Commons Attribution 4.0 International agreement unless otherwise specified and were current at the time of the event. **Files and materials included in this record:** - Event metadata (PDF): Information about the event including, description, event URL, learning objectives, prerequisites, technical requirements etc. - Index of training materials (PDF): List and description of all materials associated with this event including the name, format, location and a brief description of each file. - Schedule (PDF): A breakdown of the topics and timings for the workshop - Recommended resources (PDF): A list of resources recommended by trainers and participants - Q_and_A(PDF): Archive of questions and their answers from the workshop Slack Channel. **Materials shared elsewhere:** This workshop follows the tutorial ‘Introduction to data analysis with R and Bioconductor’ which is publicly available. https://saskiafreytag.github.io/biocommons-r-intro/ This is derived from material produced as part of The Carpentries Incubator project https://carpentries-incubator.github.io/bioc-intro/ Melissa Burke (melissa@biocommons.org.au) Bioinformatics, Analysis, Statistics, R software, RStudio, Data visualisation
ARDC FAIR Data 101 self-guided

FAIR Data 101 v3.0 is a self-guided course covering the FAIR Data principles

The FAIR Data 101 virtual course was designed and delivered by the ARDC Skilled Workforce Program twice in 2020 and has now been reworked as a self-guided course.

The course structure was based on 'FAIR Data in the...

Keywords: training material, FAIR data, video, webinar, activities, quiz, FAIR, research data management

ARDC FAIR Data 101 self-guided https://dresa.org.au/materials/ardc-fair-data-101-self-guided-bba41a59-8479-4f4f-b9ee-337b9eb294bf FAIR Data 101 v3.0 is a self-guided course covering the FAIR Data principles The FAIR Data 101 virtual course was designed and delivered by the ARDC Skilled Workforce Program twice in 2020 and has now been reworked as a self-guided course. The course structure was based on 'FAIR Data in the Scholarly Communications Lifecycle', run by Natasha Simons at the FORCE11 Scholarly Communications Institute. These training materials are hosted on GitHub. contact@ardc.edu.au training material, FAIR data, video, webinar, activities, quiz, FAIR, research data management
HPC file systems and what users need to consider for appropriate and efficient usage

Three videos on miscellaneous aspects of HPC usage - useful reference for new users of HPC systems.

1 – General overview of different file systems that might be available on HPC. The video goes through shared file systems such as /home and /scratch, local compute node file systems (local...

Keywords: HPC, high performance computer, File systems

Resource type: video, presentation

HPC file systems and what users need to consider for appropriate and efficient usage https://dresa.org.au/materials/hpc-file-systems-and-what-users-need-to-consider-for-appropriate-and-efficient-usage Three videos on miscellaneous aspects of HPC usage - useful reference for new users of HPC systems. 1 – General overview of different file systems that might be available on HPC. The video goes through shared file systems such as /home and /scratch, local compute node file systems (local scratch or $TMPDIR) and storage file system. It outlines what users need to consider if they wish to use any of these in their workflows. 2 – Overview of the different directories that might be present on HPC. These could include /home, /scratch, /opt, /lib and lib64, /sw and others. 3 – Overview of the Message-of-the-day file and the message that is displayed to users every time they log in. This displays info about general help and often current problems or upcoming outages. QCIF Training (training@qcif.edu.au) HPC, high performance computer, File systems
Basic Linux/Unix commands

A series of eight videos (each between 5 and 10 minutes long) following the content of the Software Carpentry workshop "The Unix Shell".

Sessions 1, 2 and 3 provide instructions on the minimal level of Linux/Unix commands recommended for new...

Keywords: HPC, high performance computer, Unix, Linux, Software Carpentry

Resource type: video, guide

Basic Linux/Unix commands https://dresa.org.au/materials/basic-linux-unix-commands A series of eight videos (each between 5 and 10 minutes long) following the content of the Software Carpentry workshop ["The Unix Shell"](https://swcarpentry.github.io/shell-novice/). Sessions 1, 2 and 3 provide instructions on the minimal level of Linux/Unix commands recommended for new users of HPC. 1 – An overview of how to find out where a user is in the filesystem, list the files there, and how to get help on Unix commands 2 – How to move around the file system and change into other directories 3 – Explains the difference between an absolute and relative path 4 – Overview of how to create new directories, and to create and edit new files with nano 5 – How to use the vi editor to edit files 6 – Overview of file viewers available 7 – How to copy and move files and directories 8 – How to remove files and directories Further details and exercises with solutions can be found on the Software Carpentry "The Unix Shell" page (https://swcarpentry.github.io/shell-novice/) QCIF Training (training@qcif.edu.au) HPC, high performance computer, Unix, Linux, Software Carpentry
Transferring files and data

A short video outlining the basics on how to use FileZilla to establish a secure file transfer protocol (sftp) connection to HPC to use a drag and drop interface to transfer files between the HPC and a desktop computer.

Keywords: sftp, file transfer, HPC, high performance computer

Resource type: video, guide

Transferring files and data https://dresa.org.au/materials/transferring-files-and-data A short video outlining the basics on how to use FileZilla to establish a secure file transfer protocol (sftp) connection to HPC to use a drag and drop interface to transfer files between the HPC and a desktop computer. QCIF Training (training@qcif.edu.au) sftp, file transfer, HPC, high performance computer
Connecting to HPC

A series of three short videos introducing how to use PuTTY to connect from a Windows PC to a secure HPC (high performance computing) cluster.

1 - The very basics on how to establish a connection to HPC.
2 - How to add more specific options for the connection to HPC.
3 - How to save the...

Keywords: HPC, high performance computer, ssh

Resource type: video, guide

Connecting to HPC https://dresa.org.au/materials/connecting-to-hpc A series of three short videos introducing how to use PuTTY to connect from a Windows PC to a secure HPC (high performance computing) cluster. 1 - The very basics on how to establish a connection to HPC. 2 - How to add more specific options for the connection to HPC. 3 - How to save the details and options for a connection for future use. QCIF Training (training@qcif.edu.au) HPC, high performance computer, ssh